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    • Now you can build ...

    Now you can build your own smartphone microscope

    Written by Anjali Nimesh Nimesh Published On 2017-11-28T19:22:34+05:30  |  Updated On 28 Nov 2017 7:22 PM IST
    Now you can  build  your own  smartphone microscope

    Add one more thing to the list of tasks your smartphone can perform. University of Houston researchers have released an open-source dataset offering instructions to people interested in building their own smartphone microscope.


    The researchers describe the process in a paper published in Biomedical Optics Express, demonstrating that a basic smartphone equipped with an inexpensive inkjet-printed elastomer lens can be converted into a microscope capable of fluorescence microscopy, able to detect waterborne pathogens and perform other diagnostic functions.


    Wei-Chuan Shih, associate professor of electrical and computer engineering, said fluorescence microscopy is "a workhorse," used in biology, medical diagnostics, and other fields to reveal information about cells and tissue that can't otherwise be detected. The technique allows more information to be harvested from fluid, tissue and other samples, but not everyone has access to an optical microscope that can use fluorescence.


    It could extend sophisticated imaging techniques to rural areas and developing countries, Shih said. But it also could have more widespread applications, such as allowing backpackers an easy way to test for pathogens in rivers and streams.


    "We really hope anyone who wants to build it can," he said. "All the pieces can be made with a 3-D printer. It's not something that belongs just to the lab."


    The work was partially funded with a $100,000 grant from the National Science Foundation's citizen science initiative, which encourages scientists to find ways to expand knowledge of and access to research.


    Shih's lab created an inexpensive lens that can turn a smartphone into a microscope in 2015; he and members of the lab created a company to produce and distribute the inkjet-printed lenses, which attach directly to a smartphone camera lens.


    They continue efforts to improve on that process, and in an article published earlier this fall in Applied Optics they reported engineering a platform - constructed with low-cost parts including LEGO bricks and plastic imaging components - to ensure high-throughput quality inspection of the inkjet-printed lenses.


    The lenses were used in the work reported in Biomedical Optics Express, which details how the researchers combined simple LED lighting with a 3-D printed cartridge designed to hold a conventional glass slide. The light and cartridge attach to the smartphone.


    While conventional tabletop microscopes shine light through the sample from above, the Shih lab's technology launches the light from the side of the slide, which is about one millimeter thick. The LED light travels through the glass, refracting to allow the observer to view cell nuclei and structure.


    That's both less expensive and less complicated to operate, Shih said.


    "To pursue ultra-simplicity for open-source do-it-yourself fluorescence smartphone microscopy, we report the development of an integrated single lens add-on for multi-color fluorescence imaging," the researchers wrote. In addition to Shih, those involved with the project include Yulung Sung, a doctoral student in the Department of Electrical and Computer Engineering at UH, and undergraduate Fernando Campa.


    Results from testing water samples for pathogens including Giardia lamblia and Cyrptosporidium parvum using the technology were compared with results obtained using a tabletop optical microscope. Resolution was slightly higher with the optical microscope, but the researchers reported resolution of two microns with the smartphone technology.


    Shih said he looks forward to seeing the device used by people outside the scientific community.


    "I feel more and more excited about seeing people adopt simple basic scientific gadgets," he said. "I think it will have more impact if we let people play with it, rather than trying to hold it as a secret. We should make it as easy and accessible as possible for everyone."

    Biomedical Optics Expressfluorescence microscopyLED lightssmartphone microscopeUniversity of HoustonWei Chuan Shih
    Source : Eureka Alert

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    Anjali Nimesh Nimesh
    Anjali Nimesh Nimesh
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