Biomarker present in intestine can accurately diagnose necrotizing enterocolitis

USA: A biomarker present in the human intestine can accurately diagnose necrotizing enterocolitis (NEC) -- a deadly infant disease, according to a recent study published in the journal JAMA Network Open.

The study, one of the largest prospective clinical studies in premature infants yet, involved a diagnostic analysis of 136 premature infants. It found that a protein involved in managing harmful bacteria in the human intestine can be a reliable biomarker for the noninvasive detection of NEC.

NEC is a common neonatal gastrointestinal (GI) tract emergency with a high mortality rate (as high as 50%) in neonates. It is also associated with long-term morbidities, including short-gut syndrome, nutritional deficiency, and neurodevelopmental delay. To date, no clinical test has been established as the gold standard to diagnose NEC. X-rays are used to diagnose advanced disease, but their sensitivity can be as low as 44%.

Maya Heath, Louisiana State University School of Medicine, Children's Hospital of New Orleans, New Orleans, and colleagues evaluated whether aberrant intestinal alkaline phosphatase (IAP) biochemistry in infant stool is a molecular biomarker for NEC and not associated with sepsis.

"This study exemplified academic medicine at its best," notes Sunyoung Kim, Professor of Biochemistry and Molecular Biology at LSU Health New Orleans School of Medicine and senior author. "It creates linkages between unexplained patient presentations and scientific inquiry. We were driven by the desire to build unique and useable tools to fight a disease that has been unexplained for nearly 200 years in the most fragile patient population - preemie babies."

Previous research suggested that NEC is preceded and accompanied by changes in the complex and dynamic collection of microorganisms called gut microbiota, which live in the intestine. In this study, the researchers measured and analyzed the activity of the protein, intestinal alkaline phosphatase (iAP) obtained from stool samples from the babies enrolled in the study at Children's Hospital of New Orleans, Touro Infirmary, and St. Louis Children's Hospital. Clinical data collected included gestational age, birth weight, Apgar scores, delivery type, race, gender, feeding, antibiotics, laboratory and radiology results, as well as surgical notes. Eighteen percent of the babies were classified as having severe NEC; 14% had suspected NEC, and 68% were NEC control.

Since iAP activity precedes the chemical process triggering inflammation, the researchers studied the abundance and enzyme activity of iAP shed in the stool to assess the correlation of two iAP biochemical measures with disease severity. They found that elevated levels of iAP protein linked to NEC were shed in the samples, but the proteins were dysfunctional in the NEC patients. The accuracy rates using iAP levels and iAP activity as markers for severe NEC were 97% and 76%, respectively. The accuracy values were similar for suspected NEC - 97% and 62%, respectively.

These results indicate that iAP biochemistry and abundance can be used as diagnostic biomarkers for both severe and suspected NEC. Significantly, iAP measures were not biomarkers for sepsis, another potentially fatal condition that can exhibit symptoms similar to NEC. A correct diagnosis is crucial to treatment decisions.

The biomarker has doubled the diagnostic identification of the disease, compared to the current gold standard - a milestone important at both the bench and the bedside.

"Intestinal AP is the first candidate diagnostic biomarker, unique in its predictive value for NEC," reports Dr. Kim. "It is correlated only with NEC and is not associated with sepsis or other non-GI infections. The clinical potential of this noninvasive tool lies in its use to identify infants most at risk to develop NEC, to facilitate the management of feeding and antibiotic regimens, and monitor response to treatment."

"What began as a collaboration between Biochemistry and Pediatrics at LSU Health New Orleans School of Medicine to address a life-threatening condition has grown into a multicenter national partnership," concludes Kim. "We are working hard here at LSU Health to create solutions for people in our state and to use our discoveries to help infants across the country."

More Information: "Association of Intestinal Alkaline Phosphatase With Necrotizing Enterocolitis Among Premature Infants" published in the JAMA Network Open journal.

DOI: 10.1001/jamanetworkopen.2019.14996

Journal Information: JAMA Network Open